Scientist, DNA Construction and Gene Design

The Scientist, DNA Construction and Gene Design will engage in activities associated with the design of vectors and genetic functional units across multiple eukaryotic and prokaryotic species with an emphasis on promoters, 5’ and 3’ regulatory elements, and bioactive RNA. The candidate must have extensive experience designing vectors for multiple eukaryotic species, with an emphasis on constructs that are optimized for drug development. The candidate will also assist the DNA construction team in DNA assembly, sequence verification, and tracking of key vector orders.


Job Snapshot:
Posted On:
Location:Germantown, MD
Department:Human Therapeutics
Job Type:Full Time
Education:Masters or Ph.D. in Molecular biology, Biochemistry, Genetics, Synthetic Biology or related discipline.
Experience:Strong background in mammalian gene expression and gene program design
Reference Id:1432
Travel Required:No
Manage Others:No
 
Description:
DUTIES AND RESPONSIBILITIES:
 
  • Aid in design and construction of eukaryotic DNA vectors, with an emphasis on mammalian systems.
  • Interact with stakeholders within Intrexon’s Human Therapeutics Divisions, and other groups, to execute design-build-test-learn workflow.
  • Utilize bioinformatics tools to design promoter and DNA/RNA regulatory elements aimed to control the timing, location, and amount of gene expression in multiple systems.
  • Review, analyze, and prioritize candidate regulatory units from the scientific and patent literature as well as design novel synthetic regulatory elements.            
  • Analyze and interpret bioassay data.  
  • Adopt innovative technologies or cloning methods and apply them for vector construction.
  • Create designs of proprietary DNA vector backbones, viral and non-viral vectors, and genetic modules.
  • Review design files and blueprints of genetic elements produced by partner organizations.
  • Assist in the design of experiments and associated combinatorial vector sets.
  • Propose projects to train, extend, optimize and enhance the team’s molecular biology operation capabilities.
  • Assist in writing, reviewing and editing of technical documents and reports.
 
EDUCATION AND EXPERIENCE:
 
  • Masters or Ph.D. in Molecular biology, Biochemistry, Genetics, Synthetic Biology or related discipline.
  • Strong background in mammalian gene expression and gene program design.
  • Knowledge of promoter substructure and transcriptional regulatory components.
  • Knowledge of non-coding RNA elements (e.g., IRES, ribozymes, miRNA, lncRNA, synthetic introns, stability motifs, 3’end processing motifs, translational enhancers, etc.)
  • Proficient with DNA sequence analysis tools such as CLC or Geneious.
  • Knowledge and experience in cloning and general molecular biology.
  • Knowledge or experience in viral vector (Lentiviral, Adenoviral, or AAV vectors) based gene delivery.
  • Advanced skills in technical literature search and analysis.
  • Experience in RNASeq data analysis is a plus.
  • Experience in molecular pathway/network analysis, genome annotation, and comparative genomics is a plus.
 
DESIRED KEY COMPETENCIES:
 
  • Ability to achieve in a milestone-driven, rapidly changing research environment.
  • Ability to work collaboratively with a team of scientists of diverse backgrounds and experience levels.
  • Strong personal leadership and self-direction.
  • Well-developed interpersonal skills and demonstrated ability to work with and coordinate demands from multiple customers, both internally and with partners.
  • Demonstrated attention to detail and ability to effectively manage projects and priorities.
  • Strong oral and written communication skills and an ability to communicate complex concepts in a concise, simplified manner.
  • Ability to handle a fast-paced, multi-tasking, global environment, and prioritize effectively.
  • Strong quantitative and analytical skills.
  • Demonstrates the highest ethical standards, trustworthiness and has a high degree of personal responsibility.
  • Proficiency with MS Word, Excel, PowerPoint.
EOE MFDV